Thesis Goal
E. coli is one of the most widely used hosts for production of recombinant proteins. Up to 30% of all biopharmaceuticals are still produced within E. coli, as the host offers fast growth rates and cheap cultivations. Process parameters have been optimized in fed-batch technology, and high titers can be achieved within short time of cultivation. As industry is nowadays aiming to realize a fully integrated continuous process, long-term cultivations need to be established.
However microbial systems suffer from stability in chemostat cultivations. Intracellular shifts in transcriptome and proteome lead to decreased levels of specific productivity, due to unknown reasons (Peebo & Neubauer, 2018). Within this master thesis you will get a chance to investigate these intracellular shifts in more detail and further you will learn cultivation strategies, how to possible overcome transcriptional shifts.

Chances
You will get the chance to gain comprehensive knowledge about E. coli upstream processes in fed-batch and continuous applications. Thereby you will be introduced into working with bioreactors, homogenizers, Flow cytometry and HPLC techniques.

Time-frame
Start: 04.11.2019, End: 01.05.2020 (Time-frame can be discussed on demand)

Demands
Motivated student in Biotechnology, Chemical and Biochemical Engineering, Chemistry, Bioprocess Engineering or similar with the ability to work as part of a team as well as independently.